|(E)-5,6-二氯-2-(3-(5,6-二氯-1,3-二乙基-1,3-二氢-2H-苯并[d]咪唑-2-亚基）丙-1-烯基)-1,3-二乙基-1H-苯并[d]咪唑-3-碘化物|CsnPharm

CAS No.: 47729-63-5

(E)-5,6-Dichloro-2-(3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzo[d]imidazol-2-ylidene)prop-1-en-1-yl)-1,3-diethyl-1H-benzo[d]imidazol-3-ium iodide Catalog No. CSN21697

Synonyms: T 3168

JC-1 is a cyanine dye that has been used to determine mitochondrial transmembrane potential.

规格	价格	促销价格	库存	数量

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

加入购物车大规格询价

加入收藏

产品咨询

400-920-2911

sales@csnpharm.cn

tech@csnpharm.cn

纯度 & 质量文件批次:

靶点选择性

生物活性

实验方案

技术信息

生物活性

描述JC-1 is a dye used to monitor mitochondrial potential. The monomeric form has an emission maximum of 529 nm. When used at higher concentrations or potentials, this dye forms red fluorescent J-aggregates with an emission maximum at 590 nm. The ratio of this green/red fluorescence only depends on the membrane potential of mitochondria, but not mitochondrial shape, density, or size. You may follow a protocol to label mitochondria in cultured cells with JC-1 from Cold Spring Harbor Laboratory as follow, 1. Prepare a 1-mg/mL stock solution of JC-1 in DMSO. Protect from light, and store at –20˚C. 2. Prepare a small aliquot of fresh 10 µg/mL JC-1 in the appropriate cell culture medium with 10% calf serum at 37˚C. Discard staining solutions that are older than 3 min. 3. Aspirate the cell culture medium from cells grown on a coverslip. Do not allow the cells to dry out. 4. Apply 50 µL of the JC-1-labeling solution to the cells. 5. Incubate the cells with the JC-1 labeling solution in a covered Petri dish for 10 min at 37˚C in a cell culture incubator. 6. Aspirate the labeling solution, and rinse the cells three times in cell culture medium without JC-1. 7. Mount the coverslips as described in Mounting Live Cells onto Microscope Slides (Chazotte 2011). 8. Image the cells. For the JC-1 monomeric dye, λ_excitation maximum is 514 nm and λ_emission maximum is 529 nm (using methanol as the solvent). For the J-aggregate form of JC-1, λ_excitation maximum 485–585 nm and λ_emission maximum 590 nm (using methanol as the solvent).
参考文献

[1] Chazotte B, et al. Labeling mitochondria with JC-1. Cold Spring Harb Protoc. 2011 Sep 1;2011(9). pii: pdb.prot065490.

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 1.5332 mL
- 7.6661 mL
- 15.3321 mL
- 5 mM
- 0.3066 mL
- 1.5332 mL
- 3.0664 mL
- 10 mM
- 0.1533 mL
- 0.7666 mL
- 1.5332 mL

技术信息

CAS号	47729-63-5	储存条件	粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6 月 -80°C:12月
分子式	C25H27Cl4IN4	运输	蓝冰
分子量	652.23	别名	T 3168

大规格询价

号为必填项

姓名: 邮箱: 手机号:

留言:

提交

(E)-5,6-Dichloro-2-(3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzo[d]imidazol-2-ylidene)prop-1-en-1-yl)-1,3-diethyl-1H-benzo[d]imidazol-3-ium iodide Catalog No. CSN21697

靶点选择性

请登录您的专属账户

确认信息